Optimization of In Vitro Culture Conditions of Narcissus Bulb (Narcissus tazetta L.)

Narcissus tazetta L. is one of the most important flowering bulbous plants that are traditionally propagated by bulb splitting which is a costly and time-consuming method. Therefore, the application of in vitro culture techniques can help to increase the reproduction coefficient of this ornamental plant. So, the study was conducted in two separate experiments. In the first experiment, the effect of twin scales position on the mother bulb on the regeneration of twin scales of Narcissus were investigated in MS medium containing 1mg/l BA in combination with 0.2mg/l NAA in a completely randomized design. In the second experiment, the effect of different types of auxin on rooting of Narcissus plantlets was investigated. For this purpose, ½MS medium containing 1 mg/L NAA, IBA, IAA and hormone-free medium was used. The results showed that the application of different layers of Narcissus twin scales had a significant effect on the number and diameter of regenerated bulblet. In fact, application of the inner scales of mother bulb led to an increase in the number of regenerated bulblet, but the diameter of regenerated bulblet decreased. Since the diameter and number of regenerated bulblet are both important parameters during in vitro culture conditions, it is recommended to use twin scales group one and two for propagation of Narcissus bulb. The results of this study also showed the superiority of hormone-free ½MS medium and ½ MS medium containing 1 mg/l IAA for rooting of Narcissus tissue culture plantlets.