Investigation of Azelaic acid and Potassium phosphite treatments effect on biochemical and metabolic changes of Eryngium caucasicum

In this study, the effect of exogenpous Azelaic acid and potassium phosphite on some antioxidant enzymes activity, lignin synthesis and metabolic pathways were investigated in Eryngium caucasicum. This experiment was performed in a completely randomized design in controlled consitions. Plants at the three-leaf stage were treated with potassium phosphite (2 g/L) and azelaic acid (1 mM) by spraying method. Results showed that Azelaic acid treatment led to a significant increase in the activity of SOD, POD and PAL enzymes, while potassium phosphite caused a remarkable increase in CAT and PAL enzymes but a decrease POD and SOD enzymes activity. Also, metabolic ouputs through GC-MS technique showed that the treatments had different effects on accumulation of some metabolites for example, the level of Phytol increased sharply under the influence of Azelaic acid treatment, while exogenous potassium phosphite increased n-Hexadecanoic acid and Linoelaidic acid. Metabolic cycles can be engineered with the precise use of these materials, which, in turn, can lead to the accumulation of a specific group of secondary metabolites.