Comparisonof DNA extractionmethods fromHalocnemum strabilaceum(Amaranthaceae)

Molecular techniques such as DNA extractionand DNA sequencing are playing an important role in studying the genetic makeup ofthe plant and identifying the evolutionary relationship using DNA barcoding. Extraction ofDNA from plant tissue is often problematic, as many plants contain high levels of secondary metabolites that can interfere with downstream applications, such as PCR. Removal of these secondary metabolites usually requires further purification of the DNA using organic solvents or other toxic substances. In this study, we have focused on the DNA isolation process using three isolation techniques: the cetyltrimethyl ammonium bromide (CTAB) method that uses the ionic detergent hexadecyl trimethylammonium bromide and chloroformisoamyl alcohol, BioFACT,and GeneAll methods on desert/rangeland plant includingHalocnemum. The quantityand quality of extracted genomic DNAs werecompared by employing the spectrophotometer, Nano-Drop, agarose gel electrophoresis,polymerase chain reaction (PCR) methods,and molecular markerssuch as ISSR. Our results showedthat themodifiedmethod of CTAB provided the best results than the BioFACT and GeneAllmethods for extracting DNA from the tissues of Halocnemum. We present a safe and cost-efficient DNA purification procedure andrecommend using this CTABmethod to extract DNA from plant tissues and to use the young leaf for the highest DNA yields.