NF-α, IL6, IL1-α, IL-10 and TGF-β1 Gene Expression in Iranian Acute Myeloid Leukemia Patients

Acute myeloid leukemia as one of the most common malignant disorders in adults is a heterogeneous clonal disorder of blood producing cells. The aim of this study was to analyze the expression of TNF-α, IL6, IL1-α, IL-10 and TGF-β1 gene in acute myeloid leukemia at diagnosis. The current study was conducted on 50 patients with acute myeloid leukemia and 50 subjects as control group. Serum levels of TNF-α, IL6, IL1-α, IL-10 and TGF-β1 were measured by ELISA. The RNA was extracted, and the expression of TNF-α, IL6, IL1-α, IL-10 and TGF-β1 gene was evaluated using real-time PCR (ΔΔCT computational) after cDNA synthesis. Statistical analysis was performed using Statistical Package for the Social Sciences 19, and P<0.05 was considered as significant level. In patients suffered from acute myeloid leukemia, the mean serum levels of TNF-α, IL6, IL1-α, IL-10 and TGF-β1 were lower than those in the control group. Moreover, the mRNA expression of cytokines in peripheral blood mononuclear cells had a significant decrease in AML patients compared to control group (p<0.0001). The results showed that changes in TNF-α, IL6, IL1-α, IL-10 and TGF-β1 had significant relationship with acute myeloid leukemia. Therefore, the present study supports the use of cytokines as biomarkers in the diagnosis of acute myeloid leukemia.