Optimization of fatty acids produced by Candida glabrata isolated frome soil

Oils extracted from microorganisms (SCO) are preferable to vegetable oils due to containing more gamma-linolenic acid, more stability against oxidation, and less content of residual pesticides. The oils can be used as dietary supplements. The aim of this study was molecular identification of lipid-producing yeasts and molds in soil and optimization of fat production by Candida glabrata using the Taguchi design.

Several yeasts and molds were isolated from the soil samples of groves and near oil change shops and restaurants. Molecular identification was performed by polymerase chain reaction (PCR). Among them, the highest lipid producers were selected and using the Taguchi design. The best conditions in terms of carbon and nitrogen sources as well as pH for maximum lipid production were determined. The fat obtained was examined by gas chromatography with a mass spectrometer detector (GC-MS).

Among the identified species, C. glabrata had the highest lipid production. Production of palmitoleic, palmitic, linoleic, linolenic, and stearic acid was proven in this study. Lipid production in C. glabrata and Mortierella alpina was 6.9 and10.8 grams per litre, respectively.

Due to the rapid reproduction in yeasts and their ability to produce fatty acids, C. glabrata is a suitable option for fat production.