Antibacterial Activity of Juglans regia L. Dry Husk Extract against Streptococcus mutans and Lactobacillus: An In Vitro Study

We aimed to evaluate the anti-bacterial effect of the Juglans regia dry husk ethanolic extract against Streptococcus mutans and Lactobacillus.

A quantity of 200 g of dry husk was ground into powder and sieved until no large particles were found, then mixed with 1 L of 70% ethanol, yielding 20 g of J. regia paste after ethanol exclusion by vacuum evaporator and lyophilization. The Agar well diffusion method was used to detect the antibacterial activity. Statistical analysis was carried out using Welch’s t-test (unequal variances t-test) via R software.

The J. regia dry husk extract showed statistically significant higher bacterial growth inhibition in comparison with prophylaxis pumice paste for both S. mutans and Lactobacillus.

Ethanolic dry husk extract has antibacterial activity against both S. mutans and Lactobacillus.