Study of TGFβ3 gene polymorphism and its association with growth traits in Ross broiler chicken

In this research in order to identify the part of the gene polymorphism transforming growth factor beta-3 (TGFβ3) in broiler chickens Ross, polymerase chain reaction technique and single-strand conformation polymorphism (PCR-SSCP) were performed. For this purpose randomly selection was done between 200 chicks and selected chicks were bled, and genomic DNA was extracted. To determine the quality of the DNA extracted 1% agarose gel was used. Then, by using a pair of specific primers through polymerase chain reaction (PCR) gene fragment with the size of 294 bp from parts of fourth introns and entire fifth exons of the gene was amplified, and finally to determine the bands acrylamide gel and stained with silver nitrate were used. The analysis of band patterns lead to identify three band patterns AA, AB and BB, with 0.52, 0.42 and 0.06 frequency, respectively. Two alleles A and B, with a frequency of 0.73 and 0.27 in the study population was also identified. Shannon index and effective number of alleles for the TGFβ3 gene were 0.5833 and 1.6507, respectively. To confirm the results of PCR-SSCP some samples of each genotype were sequenced directly. Compare of least squares mean different genotypes showed that the gene polymorphism TGFβ3 has significant relation with leg weight and carcass traits.