Impact of Light Quality on in vitro Potato Microtubers Formation

In this present study, the influence of three-wide spectrum light-emitting diodes (LEDs), emitting warm light AP67 (moderate blue and red and low red:far-red), AP673L (high red and high red:far-red) and G2 (high red and low red:far-red) with different colours mixing blue, green, red and far-red was used for micropropagation of three Polish potato cultivars; ‘Aruba’, ‘Oberon’ and ‘Skawa’. The planlets were grown in a growth room with a 16-h photoperiod, the photosynthetic photon flux density was set at 30 μmol m-2 s-1. The tissue culture medium applied for shoots proliferation was a standard MS-based medium, microtuberization was performed on MS medium supplemented with higher sugar content (40 mg/L-1). Light quality affected the number of microtubers (MTs) per plantlet, the volume (cm3) of MTs, the fresh weight (mg) of MTs and the width (mm) of MTs. Different light qualities did not affected morphological parameters including length (mm), perimeter (mm), and cross-section area (mm2) of microtubers, while the highest number of microtuber were detected under AP67 light quality. The morphological parameters were mostly dependent on the cultivar. The lowest number of microtubers were observed under G2 treatment. In conclusion, ‘Aruba’, as well as ‘Oberon’ with AP67 and AP673L, LED lighting systems were determined to be most suitable in terms of the number of MTs per plantlet, volume (cm3), fresh weight (mg) and width (mm) of MTs.