The culture of Lactobacillus plantarum on multi-layered bioactive scaffold containing platelet rich growth factors

Wound healing and skin remodeling occur directly after skin damage, so the use of platelet rich growth factors (PRGF) and probiotics is important to accelerate this process because of their positive effects on wound healing and antibacterial activities. Combination of above biomaterials with tissue engineering techniques led to the production of a new wound dressing. Therefore, in this study, PRGF was obtained from platelet-rich plasma and a multi-layered scaffold was fabricated by electerospining method using polyurethane (PU) fibers, PRGF and gelatin fibers. Scanning electron microscopy (SEM), tensile and water contact angle tests were performed to assess the characteristics of the scaffolds. The human Adipose Mesenchymal Stem Cells (hAMSCs) were extracted and cultured with the fibroblast cells (HU-02) as co-culture cells and Lactobacillus plantarum was cultured on scaffolds with or without PRGF to evaluate cell viability, toxicity and proliferation, then antibacterial activities of L.plantarum were examined. The result of MTT assay after 14 days indicated that PRFG and L.plantarum had significant positive effect on viability and proliferation of co-culture cells. SEM photograph illustrated adhesion and proliferation of cells and bacteria on scaffolds up to 21 days. The Agar-well diffusion test confirmed the antibacterial effect of L.plantarum on Pseudomonas aeruginosa, Salmonella typhimurium, Staphylococcus aureus, and Escherichia coli with strong inhibition zone. The current multi-layered scaffold provides the appropriate wound dressing for cell adhesion, proliferation and prevents wound infection.