Helicobacter pylori genotyping among patients refered to hospitals of Tehran using PCR-RFLP technique

Helicobacter pylori (H. pylori), infecting half of the global population, undergoes genetic diversity. The aim of this study included H. pylori genotyping among patients referred to hospitals of Tehran using PCR-RFLP technique using ureAB and ureCD genes. 

 Identifying and genotyping such prevalent and variable pathogens is challenging. To date, two versions of H. pylori have been sequenced, indicating an immense variability in its genome. To distinguish the H. pylori subtypes, the PCR-RFLP technique was used as a differentiation method. Among 37 H. pylori-positive gastric biopsies and 33 viable PCR products, ureAB and ureCD genes were selected for PCR-RFLP using HaeIII and NdeII, respectively. The resultant gel electrophoresis banding patterns were analyzed with visual evaluation. 

 PCR-RFLP standard run results were more appropriate compared to a single of each technique where a pair of H. pylori strains outlined identical banding patterns considering UreAB/HaeIII and UreCD/NdeII runs. 

 The findings permit clinicians to make precise judgments on the priority of patients' treatment, cost reduction, and mitigation of the trend of resistance emergence.