Effect of Endurance Trainings and Adenosine Treatment After Brain Ischemia Reperfusion Injury on Some Angiogenic Genes Expression in Hippocampus of Male Wistar Rats

Angiogenesis improvement treatments are effective in reduction of stroke induced organ injury. The aim of this study was to investigate the effects of an endurance training with and without adenosine consumption on some angiogenic genes expression in hippocampus of male Wistar rats after ischemia reperfusion injury.

After induction of ischemia reperfusion injury, rats were divided into three groups of ischemia (group1), ischemia with exercise (group2) and ischemia with exercise and adenosine (group3). Exercise groups performed endurance trainings on treadmill for 8 weeks (5 sessions per week) with intensity of 30m per minute. Adenosine dosage in group3 was 0/4 mg per kg. Hippocampus of the rats were isolated for gene expression analysis after trainings.

There was difference between groups 1&3 and 2&3 in HIF1α gene expression (P=0/000); in case of HIF2α, all groups were different (P=0/000); R4 gene expression was different between groups 1&2 and 1&3 (P=0/004); S2 gene expression was different in all groups (P=0/000); HMGB1 gene expression was different between groups 1&2 (P=0/011), 1&3 (P=0/000) and 2&3 (P=0/000) and significant difference was observed in all groups in AP1 gene expression (P=0/000).

After ischemia reperfusion injury, eight weeks' endurance training may be effective in improvement of angiogenesis through elevation of HIF1α, HIF2α, S2, R4, HMGB1 and AP1 genes expression and this effect is reinforced by adenosine treatment.