Assessment the effect of BiFe2O4@Ag nanocomposite biosynthesized by Scenedesmus obliquus on expression of CAD, CASP8 and p53 genes in gastric cancer cell line

The use of nanotechnology products in cancer diagnosis and treatment has gained attention. The current study was performed to synthesize BiFe2O4@Ag nanocomposite using the extract of alga Scenedesmus obliquus and to evaluate its effect on the viability and apoptotic genes expression of gastric cancer cells (AGS). MTT was performed to determine the effect of the nanocomposite on cell viability of cancer cell line (AGS) and normal cell line (HEK293) and calculation of 50% inhibitory concentration (IC50). Also, Real Time PCR was used to determine the relative expression of CASP8, CAD, and p53 genes. According to the results, at 31.25µg/mL and higher, BiFe2O4@Ag was considerably cytotoxic for AGS cells. 50% inhibitory concentration was 56µg/mL for cancer line and 134µg/mL for normal line. Also, exposure of cancer cells to BiFe2O4@Ag significantly increased the expression of the CAD, CASP8 and p53 by 3.5, 3.27, and 2.51 folds, respectively. According to the results of this study, BiFe2O4@Ag nanocomposite has proper anticancer potential against gastric cancer cells and activation of apoptotic signaling pathways is proposed as a mechanism of cell inhibition.