Biochemical assessments of effect of nefidipne on ischemia-reperfusion injury in rat ovaries via systemic administration

 Different conditions like long mesovarium and adnexal venous congestion could result in torsion of ovary and subsequently obstruction of the ovarian vessels. The aim of the present study was to investigate effects of systemic administration of nefidipine on ischemia-reperfusion injury in ovaries.

Twenty-five healthy female Wistar rats about ~250g were randomized into five experimental groups (n = 5): Group SHAM: The rats underwent only laparotomy. Group Ischemia: A 3- hour ischemia only. Group I/R: A 3-hour ischemia and a 3-hour reperfusion. Group I/N: A 3-hour ischemia only and 100 mg/kg intraperitoneal administration (IP) of nefidipine 2.5 hours after induction of ischemia. Group I/R/N: A 3-hour ischemia, a 3-hour reperfusion and 100 mg/kg IP of nefidipine 2.5 hours after induction of ischemia.

Animals treated with nefidipine showed significantly ameliorated development of ischemia and reperfusion tissue injury compared to those of other groups (P<0.05). The significant higher values of SOD, tGSH, GPO, GSHRd and GST were observed in I/R/NC animals compared to those of other groups (P<0.05). Damage indicators (NOS, MDA, MPO and DNA damage level) were significantly lower in I/R/NC animal compared to those of other groups (P<0.05).

Intraperitoneal administration of nefidipine could be helpful in minimizing ischemia-reperfusion injury in ovarian tissue exposed to ischemia.