The effect of hydrazine synthesis derivative of naproxen on histopathology of the liver and liver enzymes in Three spot Gourami Fish(Trichogaster trichopterus)

Hydrazones are an important class of bioactive drugs that have attracted much attention .One of the domains in this category of compounds is the synthesis of new hydrazone derivatives from NSAIDs, which are supposed to be more effective than the previous synthesized derivatives in terms of effect strength and severity of the complications. Many compounds of different chemical derivatives are designed and synthesized to treat various diseases, among which hydrazones are included, and their anti-cancer effects have been reported so far .The aim of this study was to investigate the hepatotoxicity profile of 4-chlorobenzolidin-hydrazine-naproxen derivatives and compare it with the main drug, naproxen. 120 fish specimens of Trichogaster trichopterus with average weight of 2.4±1 gr in 8 treatments were divided into control groups, control1 (intact), control2 (DMSO) and treatment groups receiving 10, 20, 30 mg/kg of naproxen and 10, 20, 30 mg/kg of hydrazine naproxen derivatives. These concentrations were selected according to previous works and related articles. After anesthetizing the fishes, liver tissue and liver enzymes were studied in treatment groups and the groups receiving the drug compared with control groups. The statistical results of the level of liver enzymes between control groups and treatments showed a significant difference (P<0/05). Histopathological examination of the liver tissue of the treatments indicated that the liver tissue had undergone a major change in the high doses of both drugs. The results showed that naproxen and hydrazine naproxen derivatives affect liver tissue and liver enzymes by increasing doses and increased sinusoidal spacing, and adipose vacuoles in liver.