The effect of LED lights on hairy root induction and the concentration of flavonoids in Onosma pachypoda callus by Agrobacterium rhizogenes

Message:
Article Type:
Research/Original Article (دارای رتبه معتبر)
Abstract:
Onosma pachypoda Boiss. is a medicinal plant and belongs to Boraginaceae family. Enhancement of secondary metabolites using modern biotechnological methods such as tissue culture and hairy root induction by Agrobacterium rhizogenes can be important. In the present study, hairy root induction by A. rhizogenes strain A4 on the callus of O. pachypoda was examined via dry and immersion methods under different spectral LED lights. The effect of LED lights was also investigated on the concentration of flavonoid content in inoculated callus. The results showed that the highest percentage (50%) of hairy root induction was obtained by immersing the callus in the suspension of A. rhizogenes under dark conditions. The highest flavonoid content was observed in inoculated callus under blue LED light and dark conditions. Therefore, the immersion method is a suitable method for inoculation of callus to induce hairy roots in the plant. It seems that using of different spectral LED lights also affects flavonoid content in the callus of O. pachypoda.
Language:
Persian
Published:
Journal of Plant Research, Volume:36 Issue: 3, 2023
Pages:
245 to 261
magiran.com/p2658545  
دانلود و مطالعه متن این مقاله با یکی از روشهای زیر امکان پذیر است:
اشتراک شخصی
با عضویت و پرداخت آنلاین حق اشتراک یک‌ساله به مبلغ 1,390,000ريال می‌توانید 70 عنوان مطلب دانلود کنید!
اشتراک سازمانی
به کتابخانه دانشگاه یا محل کار خود پیشنهاد کنید تا اشتراک سازمانی این پایگاه را برای دسترسی نامحدود همه کاربران به متن مطالب تهیه نمایند!
توجه!
  • حق عضویت دریافتی صرف حمایت از نشریات عضو و نگهداری، تکمیل و توسعه مگیران می‌شود.
  • پرداخت حق اشتراک و دانلود مقالات اجازه بازنشر آن در سایر رسانه‌های چاپی و دیجیتال را به کاربر نمی‌دهد.
In order to view content subscription is required

Personal subscription
Subscribe magiran.com for 70 € euros via PayPal and download 70 articles during a year.
Organization subscription
Please contact us to subscribe your university or library for unlimited access!