Effects of mineral biochar on soil properties and vegetative growth of Fortuynia bungei, Calligonum comosum, and Ziziphus spina-christi

 Although man-made biochars positively affect soil properties and plant performance, their preparation and use, especially on a large scale, are costly. On the other hand, mineral biochar can be considered an alternative method. However, there is no information about the effect of this type of biochar and its method of use on soil characteristics and plant performance.

 Effect of levels of 0 (control), 5, 12, and 25% of mineral biochar (obtained from the mine of Koohbanan city, Kerman province) in three forms of use (including mixed with surface soil, mixed with deep bottom soil, and mixed with total volume soil) on the vegetative performance of bush Fortuynia bungei, shrub Calligonum comosum and tree Ziziphus spina-christi were evaluated in a completely randomized factorial design in the nursery during four months. Cultivation operations in two stages included the germination section (without biochar) and studying the treatments' effects on the species' vegetative characteristics. The seeds were planted in pots containing 100 grams of sand. After sprouting the seedlings and their three to five leaves, one seedling was selected from each pot and transferred to larger pots containing biochar. The amount of water required was determined based on the measurement of soil field capacity. Seedling characteristics were measured at 20-day intervals for four months, and biomass production was measured at the end of the experimental period. At the end of the test period, some soil properties were examined, including organic carbon, total nitrogen, absorbable phosphorus, absorbable potassium, acidity, and electrical conductivity.

 The application of different levels and the manner of using biochar to the evaluated characteristics showed that the most noticeable effect was related to the level of biochar. In other words, although using biochar or the cultivated species resulted in varying results, the amount of biochar had the greatest effect on the evaluated characteristics. The findings showed the effect of higher levels of biochar and the mixture of biochar with the total soil volume on plant species performance (Sig.0.005). Also, the species performed differently, so C. comosum had the highest growth height (65 cm), and F. bungei had the lowest growth height (19 cm). The method of using biochar also had a significant effect on plant biomass, so the highest biomass was related to F. bungei (9.2 grams) under the influence of 25% biochar mixed with the entire soil profile. The levels of biochar or plant species alone affected soil properties. However, the combined effect of the species and the level of biochar used on soil properties was not noticeable. With the application of 25% of biochar, nitrogen (from 0.14 to 0.25%), organic matter (0.88 to 4.1%), and electrical conductivity (0.76 to 1.33 dS/m) showed the most changes (Sig.<0.001).

 By increasing biochar levels, improved soil properties and plant performance were observed. On the other hand, biochar application affects plant performance depending on species. The method of using biochar also significantly changed the performance of cultivated plants so that, considering the economic discussion of using biochar, its mixture with the entire soil profile leads to higher production than mixing biochar with surface or deep soil. However, more studies are needed regarding biochar's effect on soil properties and plant performance.