Investigating phylogenetic relationships and molecular confirmation medicinal plants Moringa balochistan using DNA barcoding method

This research was conducted to evaluate the diversity and molecular confirmation of oat gaze by DNA barcoding method. Moringa seeds were cultivated in Baluchestan province of Baluchestan province including Bennett, Kishigh, Desh, Madhadi village, Nesfaran, Condar, Tang Fanvjh entrance, mouth and seven girls. After three months, the DNA was isolated from the leaf samples and the ITS2 region was amplified and sequenced. The ClustalW sequences were combined with Mega7 software, clustering and genetic similarity and spacing. The intrinsic similarities of the Moringa Baluchistan specimens were detected in the range of 99.9%. In the present study, ITS2 did not distinguish between Balochistan's ten variants in terms of genetic variation within the group. Therefore, in order to evaluate the ability to evaluate the genetic diversity of the species, another test was performed, so that the sequences of other species of Moringa family were used in NCBI, and the Moringa sequence of the present study was compared. The Moringa-Baluchestan sample sequence with Ethiopic peregrine sequences showed homology of about 92%. The emergence in this method showed that the studied populations were divided into five groups and it was determined that the similar species were placed in the same branch. The Moringa-Baluchestan sample sequence was grouped with sequences of peregrine species in one group.Since the Moringa species in Balochistan have been introduced based on morphological keys in the Iranian flora, peregrine has been introduced. Based on the results of this study, the same was also recognized and confirmed through molecular.