Evaluation of the effect of methyl jasmonate and zno and iron oxide nanoparticles on viability of callus medicine plant of seedless barberry

In order to investigate the biomarkers of callus of dwarf barberry, an experiment was conducted in October 2017 in Birjand Agricultural College. Calluses were placed on a medium containing MS / 2 hormonal composition (TDZ-BAP) and methyl jasmonate, iron oxide and zinc oxide nanoparticles in different concentrations for eight weeks. The experiment was conducted in a completely randomized design with 14 treatments in three replications. The traits were weight, weigh weight, dry weight, biomass percentage, callus level, mass moisture content and od. The results showed that treatment (TDZ and zinc oxide PPM 100) had the highest weight. The results of orthogonal comparisons of the treatments containing BAP and TDZ indicated that the highest percentage of biomass was observed in treatments (BAP and PPM 75 nano oxide). Orthogonal comparisons between treatments containing oxide nanoparticles (iron, zinc) and methyl jasmonate containing treatments indicated a superiority of zinc (100 PPM) treatments compared to other treatments. And the results indicated a positive effect of zinc oxide nanoparticles on the viability of callus of barberry.