Suppressing Effect of Human Wharton’s Jelly Mesenchymal Stem Cell Secretomes on Oxidative Stress Induced by Breast Cancer Cell Line SK-BR3

 The main reason for treatment failure and the primary cause of breast cancer deaths is metastasis. Cancer features, such as epithelial to mesenchymal transition (EMT), invasiveness, stemness, and ability to metastasize, are significantly influenced by oxidative stress.

 The primary objective of this work was to evaluate the effects of human Wharton’s jelly mesenchymal stem cell secretomes (hWJMSCs-Se) on oxidant contents and development of the breast cancer SK-BR3 cell line and alterations in EMT markers genes after treatment.

 SK-BR3 cells received 48 hours of treatment with 10, 25, or 50 μg/mL hWJMSCs-Se. The MTT test and colony formation were used to evaluate the SK-BR3 cells’ viability and proliferation capability. By using annexin V/propidium iodide (PI) staining, apoptosis was determined. The messenger ribonucleic acid (mRNA) expression levels in genes associated with antioxidants were additionally assessed. Antioxidant enzyme activity was checked after SK-BR3 treatment with hWJMSCs-Se.

 In the hWJMSCs-Se-treated SK-BR3 cells, colony counts, and viability percentages decreased significantly with time and concentration. The treated cells displayed considerably greater apoptotic indices when compared to the control. Catalase (CAT), superoxide dismutase (SOD) activities, and glutathione (GSH) content were significantly greater in the hWJMSCs-Se-exposed SK-BR3 cells. The Vimentin gene and N-cadherin gene were significantly elevated in the treated cells, and E-cadherin and β-catenin decreased conversely.

 The present study suggests that the use of hWJMSCs in the treatment of human epidermal growth factor receptor 2 (HER2)-positive malignancies provides an innovative solution for cancer therapy. As the oxidant level and EMT pathway decreased, breast cancer cell growth was significantly restricted, and mortality increased.