Optimization of induction of hairy roots in Perilla

It is possible to treat Coronavirus disease with perilla (Perilla ferutescens (L.) Britton), a medicinal plant. The current study's objective is to examine how Agrobacterium rhizogenes induces hairy roots in Perilla to produce secondary metabolites. Three replications of a completely randomized design were used for this factorial experiment. Explant with four levels hypocotyl, cotyledon, leaf, and internode as well as bacterial strain with two levels were among the factors examined (ATCC-15834 and A4). The findings showed that the interaction effect of the factors under investigation was significant for hairy root features such as day to induction, percentage of induction, and fresh and dry weight after two months. The treatment combination of cotyledon explant infected with ATCC18534 produced the highest percentage of hairy root induction, fresh weight, and dry weight compared to other treatments. By utilizing a PCR reaction to track a portion of the rolB gene, it was possible to determine that the hairy roots were transgenic because the corresponding band was not amplified in the normal or non- transformed roots. In general, the findings of the present study indicated that the bacterial strain and explant had an impact on the induction of hairy roots in Perilla, and these findings are a necessary prerequisite for hairy root culture studies to produce secondary metabolites.