How we can prepare Hydroxyapatite Nano Crystal from Egg-Shell?

In this project we prepared Hydroxyapatite (HA) with chemical formula: Ca10 (PO4)6(OH) 2 from eggshell and di-potassium-phosphate with chemical formula; K2HPO4. Adequate amount of eggshell derived Calcium Oxide (CaO) was soaked in di-potassium phosphate solution (ratio: Ca/P=1.67) at 37 Celsius degree (˚C) for different soaking times to obtain nano-crystalline HA which was characterized by X-Ray Diffraction (XRD), Transition Electron Microscopy (TEM) and FTIR studies. The crystal grain size is very little dependent on the soaking time in di-potassium-phosphate solution and the grain sizes are almost uniform and regular in shape. The egg-shell derived HA nanoparticles could be used to make scaffolds of different shapes and pore sizes for clinical uses. This process of low temperature HA synthesis is simple, and environment friendly for the mass production of pure hydroxyapatite nano- crystalline. This process of low temperature HA synthesis is simple, and environment friendly for the mass production of pure hydroxyapatite nano- crystalline.