The effect of chronic constriction injury after a period of endurance training on the expression of TRPC1 and TRPC3 in the plantaris muscle of male Wistar rats

Due to the role of TRPC1/3 in muscle atrophy, this study aimed to investigate the effect of Chronic Constriction Injury (CCI) after a period of endurance training on TRPC1/3 gene expression in the plantaris muscle of male Wistar rats.

Thirty-two rats were randomly divided into four groups of eight each (Control, Control-CCI, Training-CCI, and Training). Finally, the rats were anesthetized, and their plantaris muscle was extracted. Gene expression was measured using Real-Time-PCR. 

The findings showed a significant decrease in plantaris muscle weight in the CCI-control group compared to the training groups (Training-CCI and Training) (p=0.001). Additionally, a significant increase in TRPC1 expression was observed in the Training group compared to the control groups (Control and Control-CCI) (p=0.001). Moreover, there was a significant increase in TRPC1 expression in the Training-CCI group compared to the control groups (Control and Control-CCI) (p=0.006). Furthermore, a significant increase in TRPC3 expression was observed in the Training group compared to the other groups (p=0.001), and a significant increase in TRPC3 expression was observed in the Training-CCI group compared to the Control-CCI group (p=0.008).

The results demonstrated a significant difference in the weight of the plantaris muscle, as well as a significant increase in TRPC1/3 expression as a result of endurance training. CCI leads to muscle atrophy and TRPC1/3 dysregulation. Therefore, the history of endurance training can modulate the expression of TRPC1/3 and prevent CCI-induced muscle atrophy.