Chicken Skin Decellularization: a Platform for Tissue Engineering

Decellularized extracellular matrix scaffolds, with a three-dimensional structure and natural bioactive compounds, can mimic the natural conditions of the body. This study aimed to decellularize, characterize, and explore the potential use of chicken skin as a scaffold in tissue engineering.

In this experimental study, the chicken skin was decellularized with a combination of physical (agitation) and chemical [ionic detergent sodium dodecyl sulfate (SDS)] methods. DNA content, histological characteristics, morphology, mechanical properties, biocompatibility, blood compatibility, swelling, water retention capacity, and contact angle of the scaffold were investigated.

The decellularization of chicken skin was confirmed through DNA content assay and tissue staining. Electron microscope images demonstrated the preservation of scaffold morphology. Besides, the mechanical strength of the decellularized tissue was largely maintained. The prepared scaffold exhibited non-cytotoxic properties and showed acceptable compatibility with blood. Additionally, the scaffold displayed promising characteristics in terms of swelling, water retention capacity, and contact angle.

Our study's findings indicate that chicken skin, as a readily available material, is biocompatible, hemocompatible, and capable of retaining moisture. Therefore, it can be considered a suitable candidate for scaffold preparation in future tissue engineering research.