The Antimicrobial Activity of Propolis Ethanolic Extract and Silver Nanoparticles Synthesized by Green Method on Gram-Positive and Negative Bacteria

The increasing resistance of bacteria to different classes of antibiotics has become an important public health concern. This study was aimed at the effectiveness of the antibacterial effect of silver nanoparticles and propolis (AgNPs@propolis) on bacteria.

A hydroalcoholic extract of propolis was used for prepare of silver nanoparticles (AgNPs@propolis). The characteristics, anti-bacterial effect and cell toxicity of AgNPs@propolis were examined in vitro.

The size of the synthesized nanoparticles was 32 to 85 nm. The AgNPs@propolis had no toxic effect up to a concentration of 200 μg/mL. Compared to AgNPs and propolis, AgNPs@ propolis showed a greater inhibitory effect on the growth of gram-positive and gram-negative bacteria. propolis as a natural substance has an inhibitory effect on the growth of bacteria.

Green synthesis of AgNPs@propolis has a low toxic effect on the cell and has a high effect in inhibiting the growth of various bacteria.