The Effect of an Aerobic Exercise course on the activity of MondoA Transcription factor and its Target genes in Skeletal Muscle of Prediabetes Mice.

Objectices:

skeletal muscles play an important role in the homeostasis of the whole body.Any dysfunction of skeletal muscles can cause problems such as prediabetes and type 2 diabetes.MondoA is a transcription factor that is activated under normal conditions by increasing the amount of glucose inside the cell and transcribes its target genes(TXNIP/ARRDC4).The purpose of this study is to investigate the effect of aerobic training on the level of MondoA transcription factor activity and the expression of its target genes in the skeletal muscle of prediabetic model rats.

Methods & Materials:

In order to induce prediabetes15 male C57BL/6 mice,(aged four weeks and weighing between 12 and 14 g)were fed with a high-fat diet for 12 weeks.After induction of prediabetes mice were divided into two groups of high fat diet(5 mice/no exercise) and aerobic training group (5 mice/10 weeks of aerobic training)The aerobic exercise protocol was performed in the form of running on a treadmill 5 days a week and 45 minutes a day.The velocity also increased from 15 to 23 m/min with moderate intensity.

The results showed a significant decrease(p<0.001)in blood glucose level and insulin resistance in the PD-Ex group compared to PD. Also exercise training decreased the relative level of expression of TXNIP(p<0.01) and ARRDC4(p<0.05)genes, the presence of transcription factor MondoA in the nucleus, and increased the expression of Glut4 protein in the cell membrane(p<0.01).

In general, aerobic exercise can be effective in lowering blood sugar by decreasing the activity of MondoA in the nucleus and increasing the presence of Glut4 in the cell membrane