The effect of foli Analysis and Identification of Essential Oil Constituents in the Vegetative and Reproductive Organs of Salvia macrosiphon Boiss. in Natural Habitat of Fars Province

This study is the first to investigate the chemical composition of essential oils in three organs (flower, leaf and stem) of Salvia macrosiphon Boiss., which grows wild and belongs to Lamiaceae family, in Fras procince. In the present experiment, 30 plants in full at flowering stage were randomly prepared from the Jahrom region of Fars province and divided into three groups of 10 and then flowers, leaves, and stems of each group were isolated for testing. Essential oil was extracted from each organ with three replications as in each repetition 200 g of plant material was used by hydro-distillation using Clevenger apparatus and analyzed by a combination of GC-FID and GC-MS techniques to check their chemical variability. The mean yields of essential oil (w/w%) in flowers, leaves, and stems were , 0.48%, 0.28%, and 0.06%, respectively. The total number of compounds identified and quantified were twenty-five in flowers, eighteen in leaves and twenty-one in stems, representing 93.1%, 93.4% and 92.2% of the total oil, respectively. Results of essential oil compound analysis illustrated that flower expressed a high content of linalool. Meanwhile, bicyclogermacrene + (E)-caryophyllene and germacrene D + bicyclogermacrene were the major compounds in leaf and stem organs, respectively. Also, results showed that , in the three studied organs, sesquiterpene hydrocarbons were the common group with the highest amountin leaves (69.1%), stems (68.0%), and flowers (34.5%). The highest level of oxygenated monoterpens was found in the flower part, represented by 27.3% of linalool. In conclusion, the plant organs of S. macrosiphon had different qualities and concentrations of essential oil. Flowers were the most beneficial organ of this species for essential oil and linalool compound productions.