Association of Mir-149C/T (rs2292832) Polymorphism and Lung Carcinoma Occurrence in the Iranian Population: A Case-Control Study

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Article Type:
Research/Original Article (دارای رتبه معتبر)
Abstract:
Introduction
Lung carcinoma (LC) is a group of anomalies associated with abnormal respiratory cell divisions. Some studies reported the possible role of the miR-499 (rs3746444) polymorphism in LCs in various societies. In the current case-control study, we decided to investigate the role of miR-149C/T variant on LC occurrence in the Iranian population.
Materials and Methods
In this case-control study, a total of 172 subjects (72 LC patients and 100 control) were genotyped by Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP) technique, for this aim, 5 ml peripheral blood sample was obtained from each subject and the genomic DNA was extracted by the salting-out method. After genotyping and data collection, the frequency of alleles and genotypes were statistically analyzed (SPSS, v.20).
Results
Since the results showed, in the frequency of all types of genotypes (Dominant, Codominant, Over-dominant, and Recessive) and associated alleles (C and T) in miR149C/T no significant difference (p >0.05) was observed in comparison between the patient group and the control group.
Conclusions
According to the findings of this study there is no significant relation between the miR-499C/T (rs3746444) polymorphism and lung cancer occurrence and this polymorphism is not a significant risk factor for lung cancer in the Iranian population.
Language:
English
Published:
Journal of Applied Biotechnology Reports, Volume:11 Issue: 2, Spring 2024
Pages:
1344 to 1348
https://www.magiran.com/p2742476