Evaluation of the immunogenicity of the PLGA-based nano vaccine of clostridium perfringens beta toxin in a mouse model

In recent years, a strategy based on nanoparticles has shown that non-denatured protein toxins can be used to enhance the appropriate immune response. The toxin does not bind to its ligand on the cell surface. The results of the nanoparticle and toxin complex show that the nanoparticles facilitate the internal release of the toxin. Clostridium perfringens beta toxin is a toxin produced by Clostridium perfringens type B and C and the most important disease is bloody diarrhea of newborn lambs. The toxin loses its lethality due to its involvement, so it becomes a toxoid. The nanoparticle used in this research is PLGA, which is one of the most developed biodegradable polymers. The purpose of this study is separate, Production and purification of Clostridium perfringens beta toxin and production of its complex with PLGA nanoparticles in order to form a non-toxic structure.In this study, beta-Clostridium perfringens type B and C toxins were isolated by ammonium sulfate precipitation and gel filtration chromatography. Purified was calculated to be 10 mg / ml. In this study, beta toxin antigen was used as a basis for the preparation of nanotoxoid candidate with nanoparticle formulation. When nanoparticles are injected into mice with beta toxin, they turn the toxin into a toxoid that has no toxicity effects, and in fact the toxin cannot bind to its receptors and reveal its effects, so the mice showed no signs of disease.