Silymarin production by hairy root culture of Silybum marianum (L.) Gaertn
Author(s):
Abstract:
Silymarin production by hairy root culture of Milk thistle (Silybum marianum L. Gaertn) was investigated using Agrobacterium rhizogenes AR15834. Hairy roots were induced by injection or inoculation of explants with A. rhizogenes. One month old hairy roots were dissected from the explants and grown in Murashing and Skoog (MS) liquid medium. Polymerase Chain Reaction (PCR) using the B gene and the B-glucoronidase (GUS) assays were used for identification of the transformed hairy roots. Flavonolignan levels in the hairy roots were determined by high-performance liquid chromatography (HPLC). Five different components were isolated; taxifolin, silychristin, silydianin, silybin and isosilybin, with the following quantities, 0.009, 0.041, 0.042, 0.007 and 0.011 mg g-1 dry weihht, respectively. Silybin was the major flavonolignan. Produced by hairy roots culture may serve as a useful system for producing silymarin or studying its biosynthetic pathways.
Language:
English
Published:
Iranian Journal of Biotechnology, Volume:6 Issue: 2, Spring 2008
Page:
113
magiran.com/p586485
دانلود و مطالعه متن این مقاله با یکی از روشهای زیر امکان پذیر است:
اشتراک شخصی
با عضویت و پرداخت آنلاین حق اشتراک یکساله به مبلغ 1,390,000ريال میتوانید 70 عنوان مطلب دانلود کنید!
اشتراک سازمانی
به کتابخانه دانشگاه یا محل کار خود پیشنهاد کنید تا اشتراک سازمانی این پایگاه را برای دسترسی نامحدود همه کاربران به متن مطالب تهیه نمایند!
توجه!
- حق عضویت دریافتی صرف حمایت از نشریات عضو و نگهداری، تکمیل و توسعه مگیران میشود.
- پرداخت حق اشتراک و دانلود مقالات اجازه بازنشر آن در سایر رسانههای چاپی و دیجیتال را به کاربر نمیدهد.
In order to view content subscription is required
Personal subscription
Subscribe magiran.com for 70 € euros via PayPal and download 70 articles during a year.
Organization subscription
Please contact us to subscribe your university or library for unlimited access!