Induction of spawning in common carp Cyprinus carpio, using pituitary extract and GnRH analogue in combination with Domperidone Abstract PDF

The effectiveness of the first Iranian made gonadotropin releasing hormone analogue, [D-Ala6 des-Gly10] GnRH ethylamide, alone or in combination with domperidone, a dopamine antagonist on spawning rate, latency period, working fecundity and embryo viability in common carp, Cyprinus carpio, was investigated. Fifty two fish were divided into 5 groups and treated intrapretoneally as follows: 3 mg/Kg b.w. of carp pituitary extract (C.P.E.) as a positive control, GnRHa alone, 10 g/Kg b.w. or in combination with domperidone, 5 mg/Kg b.w. in a single or double injections 7h apart. A group was treated with propylene glycol 0.2 ml/Kg b.w. alone and considered as control. No female ovulated in groups receiving either propylene glycol or 10 g/Kg b.w. of GnRHa alone. The spawning rate was higher in female GnRHa+domperidone (10 g/Kg b.w. +5 mg/Kg) in double injections (11 out of 12) as compared to fish which injected either with C.P.E (7 out of 16) or GnRHa + domperidone in a single injection (3 out of 12) (P<0.05). The mean working fecundity, was significantly higher for fish receiving GnRHa+domperidone in single (126214 24315) or double injections (145600 27113) compared to C.P.E treated group (52435 1224) (P<0.05). There were no significant differences for latency period or embryo viability among the groups.