Evaluation of Plasma Interleukin-8 Concentration in Patients with Prostate Cancer and Benign Prostate Hyperplasia

Prostate specific antigen (PSA) has been used as a screening test for theearly detection of prostate cancer (PC) for many years. Although the introduction ofPSA test led to a considerable increase in reported prostate cancer cases, there is stillsome controversy over the sensitivity and specificity of this marker in distinguishing PCpatients from those with benign prostate hyperplasia (BPH), the most common benignprostate condition. An attempt is made to elucidate if the plasma level ofInterleukin 8 (IL-8) could be used effectively as a marker for the detection of prostatecancer. Plasma levels of IL-8 and PSA were measured in two groups of 40BPH and PC patients using enzyme-linked immunosorbent (ELISA) and radioimmunoassay(RIA) techniques, respectively. In addition IL-8 levels in PC3 and DU145 cellline supernatants were measured by ELISA technique. The concentration ofIL-8 in the plasma of PC patients was not significantly higher than the BPH subjects.Although, a correlation between plasma IL-8 concentration and the Gleason score of PCpatients was found, no indicated correlation was detected between the concentration ofIL-8 or PSA and age of the patients in both groups. DU145 and PC3 cell lines producedand secreted IL-8 in the media. Data of this investigation collectively concludeno correlation between IL-8 concentration in PC and BPH patients.