Lymphocyte Sub-populations and Lymphocyte Activation Markers in Pulmonary TB Patients

Message:
Abstract:
Background
Tuberculosis is still a major health problem around the globe. Better knowledge of different aspects of the disease, including immunology and immuno-pathogenesis, promises better control measures. Studies regarding quantitative and qualitative changes of lymphocytes have developed our knowledge to a great extent. In this study, quantitative deficiencies of lymphocyte sub-populations and their activation markers have been evaluated.
Materials And Methods
Following documentation of smear positive pulmonary Tuberculosis in adult patients referred to Massih Daneshvari Hospital, 40 new cases without any other immunodeficiency conditions such as HIV, CRF, etc were randomly selected. Tuberculin skin test (TST) and chest radiography were performed for each case, and flow-cytometry was done from peripheral blood for lymphocyte sub-populations as CD3, CD4, CD8, CD4/CD8 ratio, CD19, CD16+56, and for Lymphocyte activation markers including CD25, CD69 and HLA-DR. Comparison was made with the historical control group in a study in same city, age as well as same flow -cytometry operator and same including & excluding criteria. Statistical analysis of data using Spearman’s rho, Mann- Whitney U, and Asymp. Sig. (2-tailed) was done.
Results
18 cases (45%) were male and 22 (55%) were female. Age distribution had a minimum of 17 and a maximum of 80 with a mean of 44.18 (19.65). Considering flow-cytometric marker correlation’s, HLA-DR had significant relation to age (P=0.002). The ratio of CD4+ cells also had significant relation to CD3, CD16+56 and HLA-DR markers. A significant difference was found only in the total of CD3+ lymphocytes by comparing PPD positive cases (>or =10mm) with PPD negative patients. The ratio of CD4+ was significantly lower than the normal population, and CD8+ was significantly higher comparing to the control population. The CD4/CD8 ratio was also significantly lower than the normal population.
Conclusion
Expression of lymphocyte sub-populations during the course of pulmonary Tuberculosis disease showed positive or negative correlation. By comparing lymphocyte sub-populations of TB patients with the control group, it is concluded that CD4+, CD8+, and CD4/CD8 ratio show significant differences that confirm significant quantitative defects in lymphocytes due to tuberculosis disease. (Tanaffos 2002; 1(4): 37-44)
Language:
English
Published:
Tanaffos Respiration Journal, Volume:1 Issue: 4, Autumn 2002
Page:
37
magiran.com/p645825  
دانلود و مطالعه متن این مقاله با یکی از روشهای زیر امکان پذیر است:
اشتراک شخصی
با عضویت و پرداخت آنلاین حق اشتراک یک‌ساله به مبلغ 1,390,000ريال می‌توانید 70 عنوان مطلب دانلود کنید!
اشتراک سازمانی
به کتابخانه دانشگاه یا محل کار خود پیشنهاد کنید تا اشتراک سازمانی این پایگاه را برای دسترسی نامحدود همه کاربران به متن مطالب تهیه نمایند!
توجه!
  • حق عضویت دریافتی صرف حمایت از نشریات عضو و نگهداری، تکمیل و توسعه مگیران می‌شود.
  • پرداخت حق اشتراک و دانلود مقالات اجازه بازنشر آن در سایر رسانه‌های چاپی و دیجیتال را به کاربر نمی‌دهد.
In order to view content subscription is required

Personal subscription
Subscribe magiran.com for 70 € euros via PayPal and download 70 articles during a year.
Organization subscription
Please contact us to subscribe your university or library for unlimited access!