DIFFERENTIATION OF CRYSTAL-CONTAINING CELLS DURING ANTHER DEVELOPMENT&

Calcium crystals are evident in many parts of anther. There is little literature about differentiation of crystal-containing cells and crystal formation during anther development. Petunia hybrid grandiflora Flower buds collected at different developmental stages were fixed and studied. The results revealed that young anthers are tetrasporangiate and in each of the four corners, the primary parietal layer externally and primary sporogenous cells internally, arise from periclinal divisions of the archeosporial cells. In the young anther, septum and connective cells contain calcium crystals as druse. At stomium, there is an arrangement of a band of 8-14 cells just beneath the epidermis which densely stain and lack visible vacuoles. During the division of sporogenous cells and the formation of crescent shaped tissue, no crystals are seen in hypodermal cells. In this stage, druse crystals become larger in connective tissue. While meiosis is occurring, the hypodermal cells of stomium are elongated, yet crystals are not observable. During pollen development, hypodermal cells degenerate and sand crystals appear. It seems that these crystals result from druse crystal.