SSRs (Simple Sequence Repeats) amplification: a tool to survey the genetic background of olive oils

Abstract The genetic traceability of single-cultivar virgin olive oil was carried out by identifying the varieties from which they were produced. The study was based on the analysis of DNA sequences, using a panel of SSRs that could provide genotype-specific allelic profiles. A reliable DNA extraction method for extra virgin olive oil has been defined, and the possibility of using this DNA for fingerprinting the original cultivar has been demonstrated. The DNA was able to amplify SSR fragments and the profile of DNA from the monovarietal oil corresponds to the profile of the DNA from the leaves of the same cultivar. The genetic background of the olive oils that is the olive varieties that have contributed to the blend is successfully recognized, as well.