Effect of Methyl Prednisolone - Clemastine Combination on the Peritoneal Adhesions in Rats

Introduction & Corticosteroids and Antihistamines have been effective in reducing adhesion formation. This study was performed to examine the effect of clemastin - methyl prednisolone combination in the prevention of intra-abdominal adhesions after lapartomy in rats. The samples included 40 healthy, male rats that were randomly assigned to the case and control groups. The rats, used in the experiment, were from the same generation and the same age, weighting about 230±20g. Each rat was anesthetized with ketamine-hydrochloride (5mg/kg). Using sterile technique, the abdominal cavity was entered via a 4cm vertical midline incision. Ten longitudinal incision, each of them 1cm in length, were incised in the internal port of their right abdominal wall and a 2×2 (cm)2 piece of peritoneum was excised from the left side. Concurrently, clemastin (50 µg/kg) and methyl-prednisolone (40 mg/kg) was injected intra peritonealy in case group. Then, 20 days later, they had a second laparatomy for assessing the grade of adhesions. At last, the findings were analyzed by Mann-Whitney and fisher exact tests (P