Screening of membrane active antimicrobial metabolites produced by soil actinomycetes using membrane models
Author(s):
Abstract:
The focus of this study was antimicrobial membrane-activity of actinomycetes isolated from soils of Iran. In this work, soil samples were collected from desert and farming zones of Northern and Central Iran. A total number of 45 actinomycetes were isolated from the soil samples. In the primary screening performed to evaluate antimicrobial activity, isolated microorganisms were analyzed in terms of their general inhibition effects to indicator strains E. coli, C. albicans, and S. cervisae. It has been found that 12 actinomycetes, were effective against test microorganisms. In the secondary screening to determine membrane-active metabolites producing microorganisms, isolates having an inhibitory effect against test microorganisms, were analyzed for membrane activity using a Rapid Chromatic Detection method. Based on color changes that are easily identified by the naked eye and recorded by UV-vis spectrophotometery, two actinomycetes had membrane-activity effect and were stored for the sake of further study and identification.
Language:
English
Published:
Archives of Advances in Biosciences, Volume:1 Issue: 4, Autumn 2010
Page:
18
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