In vivo evaluation of physiological efficacy of insulin-loaded nanoliposomes prepared for oral delivery
Author(s):
Abstract:
Introduction
Oral delivery is the most favorable route for insulin administration. The aim of this study was to generate new chitosan coated insulin nanoliposomes and then to assess the physiological efficacy of these nanoliposomes after oral administration in diabetic rabbits. Methods
Nanoliposomes with negative surface charge encapsulating insulin were prepared by reverse phase evaporation method. To prepare nanoliposomes, lecithin, cholesterol, acetyl-diphosphate and β-cyclodexterin were used. Then, nanoliposomes were coated by means of incubation with the chitosan solution. The encapsulation efficiency of prepared nanoliposomes was measured by spectrophotometry technique after dissolution of the nanoparticles. The hypoglycemic efficacy of chitosan-coated insulin nanoliposomes were investigated by monitoring the blood glucose level after oral administration to diabetic rabbits. Results
Insulin entrapment efficacy for preparation of new formulated nanoliposomes 79±0.16 were significantly higher than other formulations (p<0.05). The in vivo results clearly indicated that the insulin-loaded nanoliposomes could effectively reduce the blood glucose level in diabetic rabbits from 250±0.75 mg/dl to 125±0.98 mg/dl after 4 hours. Conclusion
The results clearly suggest that nanoliposomes may be considered as a good tool for oral insulin delivery.Language:
Persian
Published:
Physiology and Pharmacology, Volume:15 Issue: 1, 2011
Page:
57
magiran.com/p860192
دانلود و مطالعه متن این مقاله با یکی از روشهای زیر امکان پذیر است:
اشتراک شخصی
با عضویت و پرداخت آنلاین حق اشتراک یکساله به مبلغ 1,390,000ريال میتوانید 70 عنوان مطلب دانلود کنید!
اشتراک سازمانی
به کتابخانه دانشگاه یا محل کار خود پیشنهاد کنید تا اشتراک سازمانی این پایگاه را برای دسترسی نامحدود همه کاربران به متن مطالب تهیه نمایند!
توجه!
- حق عضویت دریافتی صرف حمایت از نشریات عضو و نگهداری، تکمیل و توسعه مگیران میشود.
- پرداخت حق اشتراک و دانلود مقالات اجازه بازنشر آن در سایر رسانههای چاپی و دیجیتال را به کاربر نمیدهد.
In order to view content subscription is required
Personal subscription
Subscribe magiran.com for 70 € euros via PayPal and download 70 articles during a year.
Organization subscription
Please contact us to subscribe your university or library for unlimited access!