The study of infestation to Neospora caninum using the standardized domestic ELISA in dairy cows in suburb of Tabriz

Neosporosis is a disease that is caused by Neospora caninum and is recognized by abortionin cow and neuro-muscular paralysis in hind organs in dogs. At the first, this disease wasdiagnosed in Norway in 1984. Different serological methods such as ELISA are common fordiagnosis of disease. The aim of this study was the evaluation of a domestic ELISA fordiagnosis of neosporosis in the aborted cows around Tabriz and its comparison withcommercial ELISA. In this regard, tachyzoites of Neospora caninum were lysed and coated in the wells using different dilution of coating buffer. Different dilutions of positive control sera were applied in checker board manner for designing of ELISA. The secondary conjugated antibody was diluted as manufacturer’s instruction. The results showed that using 1.5μg antigen per well and the dilution of 1:100 sera can provide the best condition. To calculate the relative sensitivity and specificity of domestic ELISA, a commercial ELISA was performed as a reference test and the cut off value was estimated via ROC curve. The best relative sensitivity and specificity obtained in cut off value of 0.30 OD. In this survey, the rate of infestation in the to Neospora caninum aborted cows in Tabriz were calculated 17.1% and 18.4% by using domestic and commercial ELISA, respectively.