Correlation of peroxidase alleles and Simple Sequence Repeat (SSR) loci in somaclonal variation of Eucalyptus microtheca F. Muell

The purpose of this study was investigation correlation peroxidase alleles and Simple Sequences Repeats (SSRs) in tissue culture of Eucalyptus microtheca. To this aim, the green twigs of 1- year-old E. microtheca seedling separated and were cultured on modified MS medium (without half-strength KNO3 and NH4NO3) with different levels of hormones α-Naphthalene Acetic Acid (NAA), Kinetin (Kin) and TDZ. As a biochemical marker, the patterns of peroxidase bands were studied in PAGE method. A dimer gene location, a tetramer locus and two epigenetic bands were observed. The genome variations were investigated using SSR molecular marker and used primers shown several polymorphisms on somaclonal plantlets. Nonparametric statistical analysis confirmed SSRs significant effects on the peroxidase alleles. But the correlation two similarity matrix was 0.18 by Mental test.