Ca Transporter gene Isolation and Analysis of Its Expression Pattern in Soybean

Some plants are naturally able to acquire nitrogen from the air through a process calledsymbiotic nitrogen fixation. In soybean, a close interaction between the root and Bradyrhizobium japonica, results in the formation of nitrogen-fixing nodules. Both partners benefit from this interaction: the bacterium gains sugar from the plant, and the plant obtains reduced nitrogen. Autoregulation of Nodulation (AON) through the expression of GmNARK in soybean leave is the main genetically-controlled mechanism that regulates nodulation. Autoregulation of nodulation (AON), is a long-distance signaling network which acts to limit the proliferation of nodules which operates to limit the proliferation of nodules Affymetrix microarray and quantitative real-time reverse-transcriptase (QRT) PCR in wild type and the GmNARK AON mutant confirmed that the expression of GmaAffx.32318.1 has been decreased in wild type related to mutant and regulated in the leaf by GmNARK in a rhizobia-independent manner. This gene (GmaAffx.32318.1) that was cloned and sequenced is predicted to encode a Ca-transporter.It has 2460 bp with the coding sequence, upstream and downstream of gene. The predicted protein included 202 amino acids and contains 5 transmembrane domains that can be act as cell receptor or carrier with 70% similarity to a heterologous gene in Arabidopsis.