The In vitro efficacy of the newly-formulated insulin-loaded nanoliposomes for delivery

Oral insulin is the most familiar and easiest way of prescribing insulin. Thus, the aim of this study was to produce newly formulated Chitosan-coated insulin nanoliposomes as well as evaluate their potential efficacy for delivery in the condition of in vitro.Nanoliposomes encapsulating insulin with negative surface charge were produced using the method of reverse phase evaporation Lecithin, cholesterol, cetyl-diphosphate and β-cyclodexterin were used for producing nanoliposomes. Then, nanoliposomes were coated with Chitosan solution using incubation. Having been dissolved, the nanoliposomes were evaluated for encapsulation efficiency using the technique of spectrophotometry. Using incubation method, the destructive effect of Peptic and tryptic on the insulin containing nano capsules was determined and compared to their effect on free insulin. Insulin entrapment efficacy for the newly-formulated nanoliposomes was significantly (p<0.05) higher (79±0.16) than that of the other formulations. In all conditions, the insulin contained in Chitosan–coated nanoliposomes was highly protected from digestive effect of peptic and tryptic as compared to the uncoated nanoliposomes which did not protect the insulin from digestion. The results clearly suggest that the produced nanoliposomes may be considered as an appropriate alternative for the purpose of oral insulin delivery.