Efficiency of early (in hatchery) probiotic administration methods against salmonella colonization in Japanese quail.

Total of 270 quail fertile eggs were set in hatchery. Experimental groups including: Negative control (neither received probiotic treatment nor challenged by Salmonella)، positive control (challenged by Salmonella but not probiotic treatment) and 7 in hatchery administration in ovo injection، spray، oral gavage، cloacal drinking (cloacal inoculation)، cloacal drinking – in ovo injection، cloacal drinking– spray and cloacal drinking – oral gavage. Each group included 15 day old chicks. All chicks were challenged by 1. 75104 cfu / ml Salmonella eneteritidis using oral gavage except negative control 36 h post probiotic treatment. 12 chicks from each group were euthanized by CO2 inhalation 24 h postchallange. Cecal tonsil aseptically collected and colonisation of Salmonella were probed using culture technique. The spray method significantly reduced Salmonella enteritidis colonization (P<0. 01). On 8th day higher BW were observed in oral group (P<0. 05). The administration methods were significantly effective on the liver and bursa of Fabricius weight (P<0. 05). In conclusion probiotic administration using spray on quails day old chicks were the most effective in prevention of Salmonella colonization.