Simultaneous Determination of the Antioxidant A.O.2246 and 2-tert-butyl-4-methyl phenol by Spectrophotometric H-point Standard Addition Method

In this paper, the H-point standard additions method (HPSAM) is evaluated for the spectrophotometric analysis of two component mixtures. Synthetic mixtures of the antioxidant bis (4-methyl-2-tert-butyl hydroxyl phenyl) methane (A.O.2246) and its phenolic intermediate (2-tert-butyl-4-methyl phenol) were used for this study. Two wavelengths of 274.8 and 290.6 nm were optimized for determination of intermediate as analyte and the antioxidant A.O.2246 as interference. This work demonstrates the advantage of HPSAM method for solving the matrix and spectra interference and shows the linear relationship between concentration and absorbance in the range of 0.4 to 80 micro molar.