The effect of two plant growth regulators on callus generation of Oryza Sativa L.var Hashemi

Rice (Oryza sativa. L.) is the world most important cereal crop after wheat and maize. Rice has 24 spicies. Oryza sativa (Oryza sativa L. 2n=2x=24) is an annual grass. It belongs the family Gramineae. In this research، seed of rice (Oryza sativa L. var. Hashemi) were inoculated on to MS medium containing various concentration of 2. 4-D: (0،1،1. 5،2،3mg/l) and Kn: (0،0. 5،1،1. 5mg/l). Dِiِِfferent part of 7 days old seedling were grown on MS medium that supplemented by different concentrations of 2 hormons. Different explants that used for callus regenerataion were root،stem،hypocotyle and epicotyle. Our results showed that explants of rice in hormonal concentration: 2،4-D (2mg/l) +Kin (1mg/l) and 2،4-D (2mg/l) +Kin (1. 5mg/l) and 2،4-D 3mg/lformed more and biggest callus. Between different explants that used، epicotyle، hypocotyle and root had more potential for callus regeneration. In this research use cross section of oryza sativa.