The effects of different concentration of NH4NO3 and MgSO4 on some physiological activities on the in vitro culture of (Dianthus caryophyllus L.) explants

Carnation (Dianthus caryophyllus L.) one of the important floricultural crops with high commercial interest throughout the world. In recent years، remarkable works have been done on various aspects of Dianthus tissue culture including genetic engineering، disease resistance and storage of plant material. case our purpose of this research is to study the effect mineral nutrition Dianthus caryophyllus L.. For this reason in a completely random plan Sterile base of Dianthus carophyllus L. were divided into groups treatment and control in the base medium MS placed. The plants were placed under the treatment of ammonium nitrate with concentrations (0، 10، 30، 40) mmol/L and treatment of magnesium sulfate with concentrations (0، 0. 75، 2. 25، 3) mmol/L and the control group that consists (1. 5، 20) magnesium sulfate and ammonium nitrate. Hence، in this research the effects of ammonium nitrate and magnesium sulfate on photosynthetic pigments including chlorophyll a، b and (a + b)، and the amount of proline were examined. The most amount of cholorophylle a،b (a+b) was detected in the group treated with 30 mmol/L ammonium nitrate and the most prolin content was seen in the group treated with 40 mmol/L. Hence، The most amount of cholorophylle a،b (a+b) was detected in the group treated wiht 3 mmol/L magnesium sulfate and the most prolin content was seen in the group treated with 0 mmol/L magnesium sulfate. The differences between control and experimental groups were significant %1. In conclusion، Results indicated that increasing of the salt in basic MS medium caus to increase physiological activities and astress condition.