Evaluation of Glutamine Syntetase Gene Role in Growth Stage of Brassica Napusat and Drought Stress Condition

In order to evaluate on Glutamine Syntetase gene expression as well as amount of protein, chlorophyll and oxidative index of TBARM the experiment has been carried out using Brassica napus (Falcon cv.) in research greenhouse condition. The drought stress treatments were included -0.5, -1, -2, -4 and -6 bar that by using of equivalent water (6.5, 6, 4.7, 3, 1.1 litr respectively) have been irrigated, also field capacity (FC) irrigation (6.8 litr) has been used as control. The drought stress treatment has been carried out after plant vernalization and transferred to greenhouse. To increase accuracy, the statistical design of Randomized Complete Block (RBC)has been used whit 4 replicates. In order to measure gene activity, chlorophyll, protein and TBARM amount leaf samples have been collected at different growth stages (stem elongation, flowering, pod stage, physiologic maturity) for all treatments. The results showed, the amount of protein and chlorophyll decreased by drought stress intensity as well as plant age. In most growth stages there were not significant different for protein amount at control, -0.5 and -1 bar drought treatments. The amount of TBARM was built up by drought stress increase. The glutamine syntetase transcript intensified by plant age increase. The gene activity was gone up by increase drought stress up to -1 and declined by higher stress. The gene activity in pod and grain was lower than its amount in leaf.