Effect of 8 Weeks Treadmill Running with or without Pistachia Atlantica Liquid Extraction on Liver ABCG8 Gene Expression and Cholesterol Level in Female Rat

Reverse cholesterol transport (RCT) is a term used to describe the efflux of excess cellular cholesterol. ABCG8 is a member of the ABCG family that play a critical role in this process. It has been reported that several factors (nutrients, fasting and physical stress) could affect on gene expression. Thus, the current study was conducted to investigate the effect of endurance exercise with or without Baneh extraction on the expression of liver ABCG8 in female rat. In this study twenty wistar rats (six to eight weeks old, 125-135 g weight) were used. Animals were randomly assigned into training (n = 10) and control (n = 10) groups and further divided into saline-control (SC), saline-training (ST), and Baneh-control (BC), and Baneh-training (BT). Training groups was given exercise on a motor-driven treadmill at 25 m/min (0% grade) for 60 min/day, 5 days/week for eight weeks. Animals were fed as the oral with Baneh extraction and saline for four week. 72 hours after the last training session and after four hours fasting, rats were sacrificed and liver total RNA isolated, after PCR procedure, ABCG8 gene expression was detected by Real-time PCR method. Statistical analysis were performed using a one way analysis of variance and significance was accepted at P < 0.05. Re-sults demonstrated that independently Baneh extraction reduce ABCG8 gene expression in liver