فهرست مطالب

  • Volume:18 Issue: 3, 2017
  • تاریخ انتشار: 1396/01/15
  • تعداد عناوین: 5
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  • Mohammad Taheri, Mohammad Moradi*, Smj Mortazavi, Shahla Mansouri, Gholamreza Hatam, Fatemeh Nouri Page 1
    Introduction
    Drug resistance to one or more antibiotics is mainly believed to be a serious threat to global public health which occurs by various mechanisms. The electromagnetic field as a nonchemical tool is capable of altering the antimicrobial susceptibility of bacteria. The aim of this study is the evaluation of the antimicrobial susceptibility of the Klebsiella pneumoniae to antibiotics before and after exposure to 900 MHz radiofrequency (RF) radiation produced by a mobile phone.
    Methods
    Antimicrobial susceptibility of the bacteria to antibiotics disks were performed after exposure to 900 MHz radiofrequency radiation. Antimicrobial susceptibility was carried out by Kirby-Bauer and the inhibition zone of each antibiotic disk was measured (as mean). Additionally, growth variations under exposure were recorded and an outgrowth curve was drawn.
    Results
    Based on the results, after 12 hours of exposure to 900MHz radiofrequency, there was observed a maximum increase in the sensitivity of K. pneumoniae and shown that radiofrequency (RF) radiation can change antimicrobial sensitivity significantly (P
    Conclusions
    The findings of the study indicated that the sensitivity of K. pneumoniae was significantly increased to the various antibiotics after 12 hours of exposure to 900 MHz radiofrequency radiation. These observations could be interpreted by the concept of non-linearity in the responses of K. pneumoniae to different antibiotics after exposure to electromagnetic radiofrequency radiation and can be useful in the management of serious infectious diseases.
    Keywords: Bacteria, Antimicrobial Susceptibility, RF Simulator, Mobile Phone
  • Negar Darvish, Negin Hadi*, Elham Aflaki, Khadijeh Salehi Rad, Ali Montazeri Page 2
    Background
    Systemic lupus erythematosus (SLE) and rheumatoid arthritis (RA) are chronic and complex autoimmune diseases, involving multiple organs. Therefore, quality of life (QoL) in SLE and RA patients can be affected. The current study aimed to compare SLE and RA patients with the healthy population in terms of health-related QoL (HRQoL) in Shiraz, Iran.
    Methods
    The present cross sectional, analytical study was performed on 100 SLE patients, 100 RA patients, and 200 healthy controls, who were referred to the rheumatology clinics (Motahari and Hafez clinics) of Shiraz University of Medical Sciences. Demographic data including age, gender, educational level, and disease duration were evaluated. The Persian-translated version of 36-item short-form health survey (SF-36) was used as a tool for evaluating HRQoL. For statistical analysis, Pearson’s correlation test, t-test, and ANOVA were performed, using SPSS.
    Results
    Comparison of SLE and RA patients with healthy controls showed that the control group obtained higher scores in 8 subscales of SF-36 questionnaire. Based on the findings, RA patients had the lowest scores between the groups. Age and disease duration had a significant negative linear correlation with HRQoL, while educational level had a significant positive linear correlation with HRQoL. Nevertheless, there was no significant correlation between gender and HRQoL.
    Conclusions
    HRQoL was lower in RA patients in comparison with the SLE group. In fact, RA patients, who suffered from body pain and disability, obtained lower scores in both physical and mental domains. Therefore, promoting the patients’ health literacy, as an empowerment strategy, plays a key role in improving HRQoL.
    Keywords: Health-Related Quality of Life (HRQoL), Systemic Lupus Erythematosus (SLE), Rheumatoid Arthritis (RA), SF-36
  • Nahid Ghotbi, Mehdi Zokai, Khaled Rahmani, Farnaz Zand Vakili, Saide Zandi, Nahid Asadi, Ebrahim Ghaderi, Majid Mansouri* Page 3
    Background
    Neonatal mortality rate (NMR) is one of the most important health indicators in the world.
    Objectives
    The current study aimed at determining the factors influencing neonatal mortality in Kurdistan province, Northwest of Iran.
    Methods
    The current population-based case-control study was conducted in 2013. Cases were neonates who passed away within their first 28 days of life, in Kurdistan. The controls were selected among neonates who were born in the closest time and place to the case group. Data were collected using a questionnaire through interviews by a trained questioner. Chi-square, Fisher exact test, and logistic regression were used to analyze the data. All analyses were conducted by SPSS version 19.
    Results
    A significant difference was observed between the neonatal mortality and the place of residence, maternal education level, maternal passive smoking, age, neonate birth weight, type of delivery, and nutrition. Maternal passive smoking during the previous year, pregnancy interval of less than 3 years, placental abruption, age, birth weight, malformations, and asphyxia were the most important factors associated with neonatal mortality.
    Conclusions
    To reduce the NMR, in addition to follow-up and application of national guidelines, other necessary factors including the regionalization of the maternal and neonatal cares, planning, and development of programs with the focus on the causes and risk factors of neonatal mortality in Kurdistan province should be considered.
    Keywords: Neonatal Mortality, Risk Factor, Case, Control, Kurdistan
  • Ghasem Jalali, Maryam Hami*, Nayereh Namaee, Maryam Salehi, Mohammad Javad Mojahedi, Boshra Hasanzamani Page 4
    Background
    Vitamin D is a lipophilic hormone that affects homeostasis of calcium and phosphorus. Recent studies have shown that vitamin D deficiency may be a risk factor for metabolic syndrome (MS). Also, metabolic syndrome is highly prevalent in renal transplant patients and it can be associated with graft survival reduction.
    Objectives
    This study aimed at investigating the relationship between vitamin D deficiency and MS in renal transplant patients.
    Methods
    This cross-sectional study was performed on 86 stable renal transplant patients in warm seasons of 2014. Metabolic syndrome was diagnosed using the modified Asian adult treatment panel III (ATP III) criteria. Patients were classified based on their serum 25-hydroxy vitamin D levels: normal (> 30 ng/mL), insufficiency (16 - 30 ng/mL), and deficiency (
    Results
    Fifty percent of the patients had MS, consisting of 48 males (55.8%) and 38 females (44.2%). The mean age of the MS group was significantly higher than the non-MS group. The body mass index in the MS group was significantly higher than the non-MS group (P = 0.001). The mean serum vitamin D level in the MS group was lower, but this difference was not significant (P = 0.914).
    Conclusions
    Although the serum vitamin D level was lower in MS patients, it was not significant. Also, due to the high prevalence of both MS and vitamin D deficiency among renal transplant patients, we did not find any significant relation between vitamin D deficiency and MS.
    Keywords: Vitamin D, Metabolic Syndrome, Renal Transplant
  • Mehdi Fazeli, Neda Zarei, Bahareh Moazen, Foroogh Nejatollahi* Page 5
    Background
    Colorectal cancer (CRC) accounts for the third most common cancer-related death in human. Wnt growth factors signaling have an important role in the CRCs’ development. Frizzled family receptor 7 (FZD7) belongs to the 10-member Frizzled (FZD) family of receptors and has an important role in triggering Wnt signaling. Antibody-therapy is an important class of modern medicine. Human single chain antibodies, which are composed of VH-Linker-VL are small antigen-binding units with unique properties, which overcome monoclonal antibodies and are applied for certain cancer immunotargeting approaches.
    Objectives
    This study aimed at determining the anti-tumor properties of 2 anti-FZD7 scFvs phage antibodies.
    Methods
    Two specific anti-FZD7 scFv antibodies were assessed for their anti-cancer effects. Flow cytometry was used to investigate the binding ability of scFvs to SW-480 colorectal cancer cell line, and SKBR3 cells, as the negative FZD7 cell line. Cell proliferation assay was performed to evaluate the growth inhibition effects of the different concentrations of anti-FZD7scFv phage antibodies (1000 to 4000 phage/cell). Mann-Whitney U test was used to compare percentages of cell growth between treated and untreated cells.
    Results
    Fluorescent activated cell sorting (FACS) analysis revealed that scFv I and II bound to 62.4% and 63.7% of SW-480 cells, respectively, whereas 6.4% and 8.3% bound to SKBR-3 cells, as the negative control. Results of MTT assays on SW-480 demonstrated growth inhibition of 53%, 63%, and 83% for scFv I (3000 phage/cell) after 24, 48, and 72 hours, respectively, while scFv II (4000 phage/cell) treatment showed growth inhibition of 33%, 47%, and 63% for the same period of time, respectively. No significant inhibitory effect for scFvs on the cell growth of SKBR-3 cells was observed (P
    Conclusions
    Targeted therapy using antibodies has been considered as a new cancer immunotherapy in the recent years. In this study we applied 2 anti-FZD7 antibodies to assess their effect on colorectal cancer cells. Results demonstrated that both scFvs bound to the SW-480 colorectal cancer cell expressing FZD7 receptor. Significant cell growth inhibition for both anti-Fdz7 scFvs after 72 hours of incubation with the optimum amounts of the antibodies was detected. The stronger potential of scFv I for inhibiting the growth of cancer cells was also obtained. The use of scFvs against colorectal cancer cells offers a therapeutic potential for these antibodies in colorectal cancer immunotherapy.
    Keywords: Colorectal Cancer, Anti, FZD7 scFv, Growth Inhibition, Immunotherapy