- Volume:3 Issue: 4, 2018
- تاریخ انتشار: 1397/07/03
- تعداد عناوین: 6
The importance of synovial adiponectin values in patients with rheumatoid arthritis in comparison with osteoarthritisPages 123-128This case control study investigated the serum and synovial fluid adiponectin levels in 33 patients with rheumatoid arthritis (RA) and 26 patients with osteoarthritis (OA) as the control group.
A venous blood sample was collected from each patient in fasting condition to measure plasma adiponectin. At the same time, synovial fluid was aspirated aseptically from the swollen knee joint of all patients and controls. Blood and synovial fluid samples were centrifuged at 5,000 rpm for 5 min, then frozen and stored at -20 °C. For analysis of data, the independent t test, Mann-Whitney test, Kolmogorov-Smirnov test, Pearson’s correlation, and linear regression analysis were utilized.
Serum and synovial fluid adiponectin levels were significantly higher in RA patients than OA subjects (P valueThe present study revealed that adiponectin levels were increased in the serum and synovial fluid of RA patients compared with OA patients, and these levels may have a significant role in the pathogenesis of RAKeywords: Adiponectin, Rheumatoid arthritis, Synovial fluid, Osteoarthritis
Is there any association between human T-lymphotropic virus type 1 (HTLV-1) infection and Behcet’s disease?Pages 129-133Both human T-cell lymphotropic virus type 1 (HTLV-1) infection and Behcet’s disease (BD) are common to a similar geographic area. Furthermore, some clinical presentations of BD and HTLV-1 infection, such as ocular lesions and neurologic involvement, are the same. The aim of this study was to assess a possible association between BD and HTLV-1 infection.
In this case-control study, the HTLV-1 infection frequency in BD patients was compared with that for the general population. The case group consisted of 68 patients with a definite diagnosis of BD who referred to a research center at Mashhad University of Medical Sciences in Mashhad, Iran. The control group consisted of 210 healthy individuals selected from the general population of Mashhad. The presence of HTLV antibodies in the sera was assessed using enzyme-linked immunosorbent assay and the seroreactive samples were confirmed by polymerase chain reaction.
HTLV-1 infection was detected in 4.41% (3/68) and 1.43% (3/210) of cases and controls, respectively; however, the difference was not statistically significant (P value = 0.16).
The prevalence of HTLV1 infection in patients with BD was three-fold higher than in the general population, which suggests an association between these two conditions.Keywords: Behcet’s disease, HTLV-1 infection, Prevalence, Case-control study
Downregulation of Drosha, Dicer, and DGCR8 mRNAs in Peripheral Blood Mononuclear Cells of Patients with Rheumatoid ArthritisPages 135-143Rheumatoid arthritis (RA) is a systemic autoimmune disorder causing irreversible joint damage. MicroRNAs (miRNAs) are post-transcriptional regulators of gene expression that degrade or translate inhibition of mRNAs. miRNAs can be used as therapeutic targets and predictive biomarkers in many disordres. This study was undertaken to investigate whether or not the expression of key elements in miRNA biogenesis, Drosha, DGCR8 and Dicer mRNAs is dysregulated in RA patients.
In this case-control study, 50 patients with RA and 50 age- and gender-matched healthy subjects participated. The peripheral blood mononuclear cells (PBMCs) were separated from the whole blood, the total RNA content of the cells was isolated and the first strand cDNA was synthesized. Quantitative analysis was performed through real-time polymerase chain reaction (PCR) using SYBR Green gene expression master mix to detect mRNA level expression of Drosha, DGCR8 and Dicer.
The expression levels of Drosha and DGCR8 were significantly downregulated in patients with RA in comparison with the healthy controls (P value = 0.043, P value = 0.000365, respectively). The expression level of Dicer was downregulated in RA patients when compared to the healthy controls, although the difference in expression was not significant (P value= 0.156). RA patients with a familial history of autoimmune rheumatic disease recorded significant overexpression of all three genes. Moreover, DAS28 was significantly correlated with mRNA exoressiom of Drosha, Dicer and DGCR8.
The data suggests that downregulated expression of Drosha, DGCR8 and Dicer mRNAs may be contributing to the pathogenesis of RAKeywords: Rheumatoid arthritis, micro RNA, gene expression, Drosha, Dicer, DGCR8
Pages 145-150Systemic lupus erythematosus (SLE) is a potentially life-threatening systemic autoimmune disease that increases the patient’s risk of mortality if left untreated. This study examined the causes of mortality over 10 years in rheumatologic hospitals as referral centers for patients from southern Iran. Medical records and death certificates of patients diagnosed with SLE as the underlying cause of death were retrospectively assessed. Patients were of similar ages (more than 16 years) and had been admitted to Hafez, Shahid Faghihi, or Namazi teaching hospital (affiliated with Shiraz University of Medical Sciences) during the 10-year period from April 2001 to March 2011. A total of 101 (3.3%) SLE inpatients (from 3026 admissions) died in the named hospitals during the study period. Approximately nine deaths had occurred per year. Among them were 85 females and 16 males (female-to-male ratio of 5.3:1). The mean patient age was 36.9±12.8 years (ranging from 16-85) on admission before death. Moreover, the mean disease duration was 5.4±5.8 years. Dyspnea, a decrease in the level of consciousness, chills, and fever were the chief complaints of the majority of patients upon admission. Infections (34.7%) and cardiovascular diseases (28.7%) were the most common causes of death. Infections followed by cardiovascular diseases are still the most frequent causes of in-hospital deaths in SLE patients. No in-hospital deaths occurred due to malignancy during this study.Keywords: Systemic lupus erythematosus, mortality, Cause of Death
Association of TYK2 rs34536443 polymorphism with Susceptibility to Systemic Lupus Erythematous in the Iranian PopulationPages 151-159Systemic lupus erythematous (SLE) is a multifactorial autoimmune disorder which affects many organs and displays various symptoms. Genetic components contribute to the incidence and development of SLE. A rare functional variant within the tyrosine kinase 2 (TYK2) gene (rs34536443) is a common genetic candidate for several autoimmune diseases, including SLE. This case control study was performed to investigate the possible association of TYK2 single nucleotide polymorphism (SNP) with a predisposition for and clinical features of SLE in the Iranian population.
Genotyping was conducted on 600 patients with SLE and 600 sex-, age- and ethnicity-matched control subjects from the Iranian population. Patient and control samples were genotyped for one SNP (rs34536443) by applying allelic discrimination real-time PCR.
Statistical analysis of the allele distribution revealed no significant association (OR = 0.67, CI: 0.38-1.17, P value = 0.163) between the rs34536443 C allele and susceptibility to SLE. The CC genotype was not detected in either the patients or controls. Moreover, the CG genotypes showed no significant association with the risk of SLE (OR = 0.66, CI: 0.37-1.72, P value = 0.15).
These findings suggest that TYK2 rs34536443 is not associated with SLE susceptibility in the Iranian population. Further investigation is required to examine the mechanisms by which polymorphisms in this gene lead to SLE developmentKeywords: autoimmunity, polymorphism, systemic lupus erythematous, TYK2
Pages 161-164Behcet’s disease is a vasculitis which presents as recurrent oral and genital aphthous ulceration, uveitis and skin lesions. Unlike other types of vasculitis, venous system involvement is a common manifestation of Behcet’s disease. Despite the high incidence of deep vein thrombosis in Behcet’s disease, pulmonary artery thromboembolism (PTE) is a rare complication. In this article, we report on a 44-year old man who experienced recurring painful oral ulcers, bilateral panuveitis, superficial phlebitis and positive pathergy which had led to a diagnosis of Behcet’s disease 12 years earlier. He developed sudden onset dyspnea, pleuritic chest pain and hemoptysis two days before admission. The patient had tachypnea and mild respiratory distress at the time of admission. CT angiography showed filling defects in the inter-lobar arteries of both lungs with sub-segmental consolidation. A lung perfusion scan showed multiple segmental and sub-segmental perfusion defects in both lungs which did not match the ventilation scan. A diagnosis of PTE was made and anticoagulation with enoxaparin and warfarin was begun. The tachypnea, chest pain and hemoptysis disappeared after three days and he was discharged after 10 days with warfarin. His disease was in complete remission at eight months after discharge.Keywords: Behcet’s disease, deep vein thrombosis, in situ thrombosis, pulmonary thromboembolism