مجله دانشکده پزشکی اصفهان
پیاپی 567 (هفته سوم اردیبهشت 1399)

In the present study, the cytotoxic effects of the combination of metformin with docetaxel and 5- fluorouracil were studied on AGS gastric cancer cell line.

In this experimental study, the cells were exposed to different concentrations of metformin (1-80 mM), docetaxel (0.6-22.5 ng/ml), 5-fluorouracil (0.045-12 μg/ml), and combination of them. The cell proliferation was evaluated using MTT assay, and the cell apoptosis was evaluated by acridine orange/ethidium bromide assay.

Metformin, docetaxel, and 5-fluorouracil inhibited cell viability of AGS gastric cancer cells in a concentration- and time-dependent manner. The half-maximal inhibitory concentration (IC50</sub>) values were decreased after treatment with metformin/docetaxel and metformin/5-fluorouracil (P < 0.050). Moreover, the combination treatments significantly increased apoptosis of gastric cancer cells (P < 0.050).

Our results showed that the combination of metformin with docetaxel and 5-fluorouracil could be effective in the treatment of patients with gastric cancer.

The most common cardioplegic solutions in pediatric cardiac surgery are Del Nido and St. Thomas. The aim of this study was to compare of the effect of these two solutions on cardiac protection, and prevention of myocardial injury during the aortic clamp time.

This randomize clinical trial study was accomplished on 60 children under 12 years who were candidates for large ventral septal defect (VSD) repair surgery at Shahid Chamran hospital in Isfahan, Iran. One of handmade St. Thomas or Del Nido cardioplegic solutions was used randomly for electromechanical cardiac arrest in each children. Quantitative levels of cardiac troponin I and creatine kinase-myocardial band (CPK-MB) was measured and recorded before and 6 and 12 hours after the surgery.

There was no significant difference between the two markers of troponin I and CPK-MB in both groups (P > 0.050). There was no difference regarding the duration of cardiopulmonary bypass (P = 0.120) and cardiac rhythm return time (P = 0.200) after removal of aortic clamp in both groups. The time of aortic clamp was significantly shorter in Del Nido group (P = 0.009).

Due to the lack of statistically significant difference in quantitative levels of troponin I and CPK-MB between the two groups, it seems that efficiency of both solutions are similar.

Coronavirus disease-2019 (COVID-19) is a type of acute viral pneumonia, which began with a sudden outbreak in Wuhan, China. Although closely related to severe acute respiratory syndrome, severe acute respiratory syndrome (SARS) and Middle East respiratory syndrome (MERS), the disease excessive transmission rate distinguishes it from other viral pneumonia diseases. The pulmonary complications and infections caused by this virus, as well as the lack of specific therapeutic have caused one of the most complicated epidemics of the last century. In this review article, we attempted to discuss and describe the latest findings of immunopathogenesis, as well new and applicable treatments for coronavirus disease.